Coding

Part:BBa_K199144:Design

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-04-09)


Lux positive feedback loop (CCACU 1-SAT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 488
    Illegal AgeI site found at 1431
    Illegal AgeI site found at 1543
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 135


Design Notes

n/a


Source

LuxI from V. fischeri optimized for E. coli plus AsiSI and AscI restriction sites plus CCACT

References